Medical technology

Purdue University Researches Benefits of Fatty Acids Found in Meat

Mon, 14 Oct 2024 20:14:47 GMT

The impact of arachidonic acid, an omega-6 polyunsaturated fat found only in animal products, upon human health remains mostly misunderstood, according to an article released by Purdue University. Researchers aim to study the subject further.

Led by James Markworth , assistant professor of animal sciences , the team will carefully test the health effects of omega-6 in laboratory experiments. The U.S. Department of Agriculture’s National Institute of Food and Agriculture , will fund the research and the experiments will clarify which omega-3 fatty acids found in fish oil and seafood are responsible for yielding their health benefits.

“These polyunsaturated fatty acids are essential because you need to acquire them through the diet,” Markworth says. “They can’t be made in the body. And in particular it’s the long-chain versions, which are found in products of animal or marine origin, that are thought to potentially influence human health.”

Both omega-3 and omega-6 are long-chain, polyunsaturated fatty acids, and some of these fatty acids are also essential fatty acids.

The long-chain omega-6 fat arachidonic acid is found only in meat, poultry and eggs. “You can’t get it from vegetable sources, and you can’t get it from fish. We think that these nutrients found in meat and poultry products might have similar benefits as, say, fish oil or fish products. And that’s something you don’t hear very often,” Markworth says.

Previous research has well established that fish oil fatty acids have metabolic benefits. But which fatty acids convey those benefits and how remains unclear. The major ones are eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).

Collaborating with Markworth on the project are: Tzu-Wen Cross in the College of Health and Human Sciences , along with Tim Johnson and Kolapo Ajuwon , both in the College of Agriculture ’s Department of Animal Sciences .

“What we’re suggesting is when you eat these lipids in the diet or dietary supplements, the systemic response your body has might depend on the resident microbes first encountered in the gastrointestinal tract,” Markworth says. “And we’re proposing that the systemic response is largely mediated by the effect on the skeletal muscle.”

Markworth notes the skeletal muscle determines metabolic health, obesity and diabetes as it is the largest site of glucose disposal and insulin sensitivity.

Read more here .

Livestock and mRNA Vaccines: What You Need To Know

Fri, 27 Sep 2024 01:56:42 GMT

As misinformation regarding the use of mRNA vaccines in livestock filter through social media, there are facts begging to be set straight.

Recently, a claim was made saying producers are required to inject livestock with mRNA vaccines.

According to USDA spokesperson, Marissa Perry says, “There is no requirement or mandate that producers vaccinate their livestock for any disease. It is a personal and business decision left up to the producer and will remain that way,” in response to the claim, Associated Press shared in an article .

National Pork Board’s Director of Consumer Public Relations, Jason Menke echoed the statement to AP, noting that the decision to use vaccines and other medical treatments to protect animal health and well-being are made by the farmer under the direction of the herd veterinarian.

To further explain mRNA vaccines and shed light on controversies, Dr. Kevin Folta, a molecular biologist and professor at the University of Florida, shares his viewpoint and experience with the technology.

What are mRNA Vaccines?

First introduced to the population through the COVID-19 vaccines, mRNA (messenger ribonucleic acid) vaccines have been in development for decades, says Folta in a recent AgriTalk segment.

He adds that the technology’s potential in human health makes it a likely candidate to have a place in animal health as well. However, “the technology is being maligned in social media, and is now shaping decisions at the level of state legislature,” Folta says. This leads to the growing importance that producers and consumers become more educated on the topic.

What Folta believes began in January of this year, based on claims with very little data, certain advocates against mRNA vaccines are concerned that mRNA vaccines are in use and development in livestock. Additionally, these vaccines may then be present in the food these animals provide.

Why mRNA Vaccines Are Not Present in Food

“It’s not in your food. It’s a vaccine for the animal that, just like any vaccine, protects the animal from disease,” Folta says.

Current mRNA vaccines being used in swine are injected into the muscle, Folta explains, which causes the development of the immune response protein to then stimulate the body to work against the virus.

“In the absence of the virus, it’s kind of like giving the virus or giving the body a ‘wanted’ poster that says, ‘when this individual comes along, and this virus comes along, work against it,’ and it’s all gone within hours,” he adds.

The mRNA never leaves the cells from where it was injected. RNA is a very unstable molecule that must be kept cold, buffered and in solvent, to remain viable, Folta explains.

Additionally, any licensed vaccine comes with a minimum time before that animal can enter the food chain, also known as the “withdrawal time,” says Alan Young, professor in the Department of Veterinary Biomedical Sciences at South Dakota State University and founder of protein platform (non-mRNA) vaccine company Medgene.

The Animal’s Genes Are Not Altered

While mRNA vaccines include genetic code, Folta says the use of a mRNA vaccines does not alter the animal’s genes in any way.

“This [mRNA] is an intermediate between the gene itself and the products that the gene encodes. So, it’s like having a blueprint and a house. The mRNA is like the construction worker. It takes the blueprint and manufactures the house. In the case of the cell, it takes the DNA blueprint and then takes a little bit of that information to build part of the final structure. The mRNA is just that intermediate, it does not change the genes. It doesn’t change the DNA itself,” he explains.

What are the Benefits of mRNA Vaccines?

More flexibility and faster response to new disease, Folta describes as reasons why mRNA vaccines are becoming more popular.

Traditional vaccines require large amounts of a virus to be raised and purified before being injected to elicit an immune response, he adds. Meanwhile, mRNA encourages the body to make a little piece of protein to elicit the desired immune response.

“It’s much cleaner, much easier. If you’re moving parts in this machine, to make this product that induces an immune response, it’s so good in so many ways,” Folta says.

In pork production specifically, researchers are working with mRNA vaccines that will work this way against porcine reproductive and respiratory syndrome (PRRS), which is a viral disease that causes economic loss totals around $664 million per year in the U.S. (Holtkamp et al., 2013).

Additionally, the use of mRNA technology adds another tool to the toolbox, which may be helpful in combating diseases, such as African swine fever (ASF), avian influenza and other food-animal diseases.
“This stands to be a revolutionary technology if we don’t get in the way,” Folta adds.

Are There Risks to mRNA Vaccines?

Folta says everything has some sort of risk, but it’s important to weigh the benefits against the risk.
As seen with the COVID-19 vaccines, in rare cases, people experienced side effects from the vaccine. However, Folta is encouraged by the initial results in livestock.

“If you look in animals where these [vaccines] have been used, there have been no unusual effects noted. Everything potentially has risk, but it’s monitored, and especially in large animal populations, we can look very carefully at that for surveillance,” he explains.

mRNA Enters State Legislation

While some consumers spread misinformation about the use of mRNA vaccines, the ideas have also crept into state legislation.

The Missouri House Bill 1169 , with a special hearing set for Apr. 19 on the matter, aims to require a label be used on meat from animals treated with an mRNA vaccine, identifying the “potential gene therapy product.”
This bill falsely claims that mRNA vaccines would modify the genes of the organism, Folta explains.

mRNA vaccines are simply another modality that can protect animal health, which results in healthy animals producing the best and safest food products, Folta says, and provides producers with more options to help combat disease.

“To have affordable food, we need to have continual innovation in the animal, medical, veterinary space and mRNA vaccines are safe and an effective way to treat the animal that does not change the final product,” he adds.

The COVID-19 pandemic simply “broke the seal” to the development of these new modalities that will change the way human and animal diseases will be treated in the years to come.

More on Vaccines:

Genvax Technologies Secures $6.5 Million to Advance Novel Vaccine Platform

Cattle Veterinarians Have New Vaccination Guidelines

Don’t Assume That Old Refrigerator Is Good Enough To Store Vaccines

OTC Livestock Antibiotics Will Require Prescription June 11

Iowa State University Advances Veterinary Diagnostics with High-Volume Testing Innovation

Thu, 29 Jun 2023 12:19:52 GMT

Iowa State University’s Veterinary Diagnostic Laboratory (VDL) is set to revolutionize molecular diagnostic testing with the introduction of a cutting-edge machine—the “SmartChip.” This innovative device can hold over 5,000 samples on a plate no larger than a postage stamp and uses quantitative polymerase chain reaction (qPCR) testing technology, combined with a 384-sample system featuring automated handling features, to significantly enhance its testing capacity, says a recent release .

“The historical patterns of pathogens are changing, so we need to be prepared for risks we haven’t seen before. Having this high-throughput capability will allow us to meet industry needs, providing more cost-efficient diagnostic tests as the need for testing grows,” says Rahul Nelli, a research assistant professor of veterinary diagnostic and production animal medicine, in the release.

To refine the use of high-volume testing methods, the VDL recently secured a nearly $1 million grant from the U.S. Department of Agriculture’s Animal and Plant Health Inspection Service (APHIS). The project, funded by the American Rescue Plan Act of 2021, aims to prepare for future disease outbreaks. Through this initiative, researchers will ensure the accuracy and integration of the novel high-volume testing methods with existing systems for tracking and reporting test results.

“ISU VDL’s first-hand experience in responding to pandemics of high consequence to both animal and human health over the past few years, such as highly pathogenic avian influenza and COVID-19, have clearly illustrated the value of high-throughput testing platforms and need for further innovation,” said Dr. Rodger Main, ISU VDL director, in the release.

The SmartChip testing relies on microfluidic technology to detect targets of interest in samples, using a volume 100 times smaller than the VDL’s standard 96-well machines, the release explains. With samples precisely distributed in the chip’s 5,184 testing wells, the SmartChip machines can produce up to 30,000 test results per day. In comparison, the conventional 96-well method yields approximately 2,000 tests. Additionally, the 384-well machine offers a more moderate increase in capacity, capable of handling about 9,000 tests per day through smaller samples and automated loading.

Nelli envisions the SmartChip testing as a reserve resource for sudden spikes in demand for qPCR tests. This reliable method detects trace amounts of genetic material, including infectious agents in humans and animals. On the other hand, the 384-well automated machines can be part of regular lab operations, mitigating labor shortages. Moreover, by making test prices more affordable, they pave the way for wider use of surveillance testing among livestock producers.

Over the next two years, researchers will focus on integrating the new testing machines with existing reporting software. They will also validate the new methods and develop protocols for various samples used in veterinary diagnostics, including fluids, fecal matter, eggshells, and feathers. By 2025, these high-capacity testing methods could potentially be implemented in the VDL.

Main emphasizes in the release that ISU VDL, with the largest veterinary diagnostic laboratory caseload in the nation, plays a crucial role in serving the needs of 21st-century food animal agriculture. Consequently, the next-generation high-throughput testing platforms will undeniably contribute to fulfilling these requirements.

To Serve Greater Purpose: Pig Organ Donation Explained

Wed, 31 Aug 2022 17:47:04 GMT

From hearts and lungs to livers and kidneys, over 100,000 U.S. men, women and children currently await an organ transplant, while 17 people die each day when their need is not met, according to the Health Resources and Services Administration .

Over the past few decades, pigs have played a vital role in medical research to “fill the gap” in available organs.

In late 2021, a pig kidney was transplanted into a human without triggering immediate rejection for the first time. For three days, the new kidney was attached to a brain-dead patient exhibiting signs of kidney dysfunction. During the trial, the recipient’s symptoms showed improvement.

Only a few months later, a 57-year-old Maryland man, David Bennett, received the first heart from a genetically modified pig . Though a breakthrough in medical history, Bennett’s story ended two months later. This proved to be the longest a recipient has survived after experimental xenotransplantation .

With thousands of pigs born every year in the U.S., it might seem disappointing that more medical advancements have not been made.

However, it’s not nearly as simple as taking any pig from a farm and harvesting its organs for transplant. The preparation and care begin long before the pig is born.

First, future donor pigs receive the gene-editing tool CRISPR to “humanize” their organs as a number of genes are added or deleted.

After genetic modifications, the pig embryos are transferred to a surrogate sow inside a designated pathogen-free (DPF) facility, including filtered air, UV-treated water, concrete surroundings and sterile tools and equipment.

The risk of disease and other pathogens, such as porcine cytomegalovirus (pCMV), remains a priority when delivering the piglets. Therefore, a few days before expected delivery, surgeons perform a caesarian section. The piglets are then taken directly from the womb to a bath of disinfectant and are raised in isolation boxes, away from their mothers.

Pigs are fed a milk replacement by technicians in full biosecurity suits and are checked regularly for viruses. Those testing positive are killed.

After around six months of age, when pigs have reached adult human size, the organs are ready to be harvested and transplanted.

Despite all of the biosecurity precautions, Bennett’s fate proved disease is still possible when he contracted pCMV. The donor pig was screened four separate times before transplantation via nasal swab and polymerase chain reaction (PCR), testing negative every time. Bennett’s surgeon Bartley Griffith believes the pig was carrying a dormant pCMV infection that reactivated in Bennett’s immunosuppressed body. Though the medical team successfully treated the infection, the virus had likely already done too much damage to Bennett’s heart, a Slate article reports.

The ability to ensure a “clean pig” continues to be a challenge moving forward in finding the solution for organ transplant needs across the country.

Through Bennett’s unfortunate ending, Dr. Griffith told the New York Times, “Knowing it was there, we’ll probably be able to avoid it in future.”

New Veterinary Toxicology Training Program Created at K-State

Wed, 27 Oct 2021 13:21:05 GMT

Rapid response to animal health emergencies has prompted the creation of a new veterinary toxicology training program at Kansas State University. A $248,000 U.S. Department of Agriculture grant will enhance the ability of researchers in the College of Veterinary Medicine to answer calls for help.

The goal of the program, developed by Steve Ensley, clinical veterinary toxicologist, and Bob Larson, professor of production medicine, is to create impactful and innovative outreach tools. This will better enable livestock veterinarians to recognize and address toxicology problems in food animal species, especially cattle, small ruminants and pigs.

This project will utilize veterinary telemedicine and other distance-based education resources, including a toxicology call-in hotline for practicing veterinarians called CONSULT — Collaborative, Online, Novel, Science-based, User-friendly, Learning Tool — for common livestock toxicology problems, and YouTube training videos.

The nationwide call-in service to address common food animal toxicological emergencies was identified as a priority by the researchers.

“The toxicology section at the Kansas State Veterinary Diagnostic Lab and I receive multiple calls each day dealing with questions about food animal veterinary toxicology from across the U.S.,” Ensley said. “Many questions are about current cases that veterinarians are dealing with and they want assistance in answering specific questions. Because of the infrequent nature of most toxicological case presentations, many practicing veterinarians find it difficult to maintain the current knowledge necessary to quickly address specific toxicological emergencies.”

The outreach portals created with the grant provide new and valuable resources to practitioners.

“This program will greatly enhance currently available toxicology resources for teaching veterinary nurses and veterinary students during the last two years of their professional education,” Larson said.

Some of the resources can be modified to be content-appropriate to introduce important animal health concepts to high school students in grades 11 and 12, he said.

Outreach portals for the training materials will include the websites for the Beef Cattle Institute, Kansas State Veterinary Diagnostic Laboratory, K-State Veterinary Medical Continuing Education and the Colby Community College veterinary nursing program.

7 Steps to Create a Biosecurity Plan

Fri, 13 Nov 2020 02:32:19 GMT

No matter what type of livestock operation you run, biosecurity should always be at the forefront of your mind. Putting biosecurity protocols in place can help reduce the risk of disease being transferred to not only livestock, but to humans as well.

According to Joe Armstrong, DVM, University of Minnesota Extension cattle production systems educator, having a biosecurity plan can help protect your farm from external pathogens and can minimize the transmission of diseases on your operation. To build a biosecurity plan for your farm, Armstrong provides these seven steps.

1. Determine your goal.

Before you can develop your plan, it is important to determine your end goal. You can’t get to where you are wanting to go unless you know where you are at. To do this, Armstrong suggests asking yourself two questions:

Is there a specific disease that you are looking to target that you already have?
Is there a particular disease you are worried about acquiring?

If you don’t know the answers to these questions, that’s okay. Reach out to your veterinarian for help.

2. Develop your team.

One of the most valuable members to have on your farm’s team is your veterinarian. When formulating your biosecurity plan, be sure to include them in on the discussion.

“Your veterinarian is one of the only people you work with that can comment on your entire system and how everything works together,” Armstrong says. “They have specific biosecurity training that can help you develop a plan that targets your most significant transmission risks.”

3. Formulate the plan.

As you begin to write down your farm’s intentions, it is crucial that you be as specific as possible. No matter how simple a protocol may be, you still need to have it in writing. Armstrong suggests creating visible materials that can serve as a reminder to you and your staff.

4. Get everyone on board.

“Biosecurity only works if everyone follows the protocols,” Armstrong says. “One person that isn’t on board can derail the whole thing. Make sure everyone understands what to do and make sure everyone understands why it is important.”

Consider having a team meeting to go over the new protocols and ask employees for their suggestions and feedback.

5. Start the plan.

Now that the plan has been given the green light, it’s time to put it in action.

“The sooner it is in place, the sooner you can refine the protocols and identify problem areas that need to be resolved,” Armstrong says.

6. Fine tune.

Because of workforce turnover and changing conditions on dairy farms, biosecurity training needs to on-going and continually reinforced. It may be helpful to ask your veterinarian to attend these training sessions to answer some of the questions your team members might have.

7. Evaluate and make adjustments.

One of the most important steps in formulating a biosecurity plan is to make adjustments as necessary. Be sure to record incidences of diseases to help measure whether you plan is working or not

“If your plan has been given enough time to work, you can decide if you need to change your plan based on your evaluation,” Armstrong says.

Life-Saving Find: How This Missouri Soil Unearthed A Golden Medical Discovery 75 Years Ago

Take a step onto Sanborn Field at the University of Missouri–Columbia, and it’s a step into history.

“I’m walking in some big footsteps here,” says Tim Reinbott, the director of Sanborn Field at the University of Missouri.

Sanborn Field is the third-oldest continuous research farm in the world, but the oldest west of the Mississippi River. Nestled on the eastern edge of campus, buildings and housing have sprouted all around the field, but it’s still the root of significant scientific discoveries that are benefiting farmers and ranchers.

“We probably got more treatments than anybody else,” Reinbott explains. We have had continuous treatments for 135 years, and we’ve learned so much. And this is where so many of our common agricultural practices all started from the dollars that we gained here.”

One of the biggest breakthroughs happened 75 years ago, as the soil became the foundation of medicine still used today in humans and livestock.

“One of the interesting facts that is often overlooked is that in any soil you will find antibiotics, because it’s just the nature of how these bacteria survive in nature,” says Bob Kremer, adjunct professor of soil microbiology at the University of Missouri (Mizzou).

An Important Plot

Seventy-five years ago, Plot 23, which is still located within Sanborn Field at the university, became home to a groundbreaking discovery.

“We have to keep in mind that that was the age of the antibiotic discovery in the United States and worldwide,” Kremer says.

“Seventy-five years ago, we had known about penicillin. We had known other types of antibiotics, but they were only about 40% effective,” Reinbott explains.

“And they were looking for that golden antibiotic, that one that would really be very effective and be taken orally, not by injection.”

With so much effort to find microorganisms that could produce an antibiotic that wasn’t just effective but not toxic to humans or animals, researchers at Sanborn Field were on a mission.

“Benjamin Duggar, a gentleman that was a former faculty member here was working for Lederle Laboratories at the time, and he knew the director of Sanborn Field, who was William J. Albrecht, who was the soil microbiologist. Mr. Duggar asked him for some soil samples from Sanborn Field, that he could begin to culture for these microorganisms for some sort of an antibiotic that would serve those purposes.”

A Solution in Soil

The soil contained a golden mold that suppressed the growth of many microorganisms, including streptococci, a bacteria that causes various types of infections. From the sample, researchers eventually created aureomycin, which proved to be an antibiotic effective against 90% of bacteria-caused infections in humans.

“Dr. Albrecht then directed and assisted in collecting soil samples from Sanborn Field, which included plot 23 here, which is in continuous Timothy (grass) that had no fertilizer or manure amendments since it was established in 1888,” Kremer says.

“He knew that this plot right behind us (plot 23), that had been for 60 years managed the same way, was a perennial crop, but had no inputs. He knew we should have a lot of biology but a pretty hostile environment for them. If any place could have an antibiotic, it’s going to be here,” says Reinbott, as he stands in front of the birthing ground of aureomycin.

It was later that year, in 1945, they made the big discovery — one that proved to be a breakthrough in the medical world, for both humans and animals.

“They called it aureomycin, and ‘aureolus’ is the Latin word that means a golden color,” Reinbott adds. “It’s an antibiotic that’s been used for decades, and it’s still being used in animals. It’s also the best treatment there is today for Rocky Mountain spotted fever.”

Reinbott says for the first 30 to 40 years after the discovery of aureomycin, it was the go-to antibiotic for human medicine, but it also grew in popularity within animal medicine.

“The aureomycin antibiotic and the tetracycline cyclin class are still very useful today, because of their mechanism of action. They attack protein synthesis in the cell of these bacteria, and (are) effective on these rickettsia diseases, like the Rocky Mountain spotted fever. Because those particular bacteria are able to infect and inhabit deep within the tissue of a human being, they also do not become resistant,” Kremer says.

Life-Saving Treatment

First discovered at the end of 1945, aureomycin underwent clinical trials and was then approved by FDA to address human ailments, starting in 1948. One of the first recipients was a young boy, Tobey Hockett, who at the time, lived just outside of Washington, D.C.

“I was born Sept. 11, 1943,” says Hockett, who is now retired and lives in Florida. “Sometime around early 1949, I got a real bad stomachache. My parents did not pay attention, and it got worse and worse before they finally rushed me to the hospital. It turns out that I had peritonitis. I had a ruptured appendix.”

Hockett says the doctors didn’t give his parents much hope for his recovery, but the doctors wanted to treat Hockett with an antibiotic that had recently been developed, which was aureomycin. He remained in the hospital for one month.

Hockett says Mizzou’s discovery 75 years ago not only saved his life, but he went on to become a defense attorney and worked to save other lives. He took on death penalty cases and helped clients through drug court.

“You can’t express thanks in words,” Hockett says. “The only thing I think about is what I’ve been able to do with my life as a result of surviving that.”

Soil Sample Goes To Smithsonian

To celebrate the discovery of aureomycin, a soil sample from Plot 23 was sent to the Smithsonian Institution where it still resides today.

The discovery at Sanborn Field wasn’t just a breakthrough for human medical science, it was also a breakthrough for livestock.

“It’s very interesting, because they discovered that it was very useful not only to prevent some of these infections, but it also is a growth promoter,” Kremer says. “In the early 1950s, they discovered that chickens, for example, grew two and a half times quicker than the traditional feed that was being used at the time.”

Aureomycin is still widely used in cattle today. Such an antibiotic discovery is estimated to cost $1.5 billion in 2023 dollars.

“We’ve more aureomycin discoveries out here, we’ve just got to look for them,” says Reinbott, standing at Sanborn Field. “It may or may not be an antibiotic, but it can be something just as groundbreaking, and that’s what gets me excited.”